Methods of multilocus enzyme electrophoresis for bacterial population genetics and systematics.

نویسندگان

  • R K Selander
  • D A Caugant
  • H Ochman
  • J M Musser
  • M N Gilmour
  • T S Whittam
چکیده

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منابع مشابه

D A Caugant , H Ochman , J M Musser , M N Gilmour systematics . for bacterial population genetics and Methods of multilocus enzyme electrophoresis

extract electrophoresis, outer membrane protein electropho-resis, and various types of carbohydrate, lipid, or other chemical profiling or fingerprinting, have been used to type or characterize strains in bacterial systematics and epidemiology. However, because these methods detect phenotypic variation that is difficult, if not impossible (given the present state of knowledge of the genetic bas...

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Genetic diversity and genetic relationships among 42 Pseudomonas stutzeri strains belonging to several genomovars and isolated from different sources were investigated in an examination of 20 metabolic enzymes by multilocus enzyme electrophoresis analysis. Forty-two distinct allele profiles were identified, indicating that all multilocus genotypes were represented by a single strain. All 20 loc...

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Population structure of pathogenic bacteria revisited.

This minireview summarizes the historical development of bacterial population genetic concepts since the early 1980s. Initially multilocus enzyme electrophoresis was used to determine population structures but this technique is poorly portable between laboratories and was replaced in 1998 by multilocus sequence typing. Diverse population structures exist in different bacterial species. Two dist...

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Population structures in the SARA and SARB reference collections of Salmonella enterica according to MLST, MLEE and microarray hybridization.

In the 1980's and 1990's, population genetic analyses based on Multilocus Enzyme Electrophoresis (MLEE) provided an initial overview of the genetic diversity of multiple bacterial species, including Salmonella enterica. The genetic diversity within S. enterica subspecies enterica according to MLEE is represented by the SARA and SARB reference collections, each consisting of 72 isolates, which h...

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We studied the genetic structure of 176 bacterial isolates from nodules of Medicago sativa, M. lupulina and M. polymorpha in fifteen sites distributed in three localities in Mexico. The strains were characterized by multilocus enzyme electrophoresis, plasmid profiles, PCR restriction fragment length polymorphism of 16S rRNA genes and of the intergenic spacer between 16S and 23S rRNA genes, and ...

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عنوان ژورنال:
  • Applied and environmental microbiology

دوره 51 5  شماره 

صفحات  -

تاریخ انتشار 1986